實驗目的:研磨破碎大鼠腳掌
實驗原理
大鼠腳掌因為有皮膚及毛發(fā)加上含有一些筋,韌性很強,所以難以破碎,需配合液氮研磨才能得到較好的研磨效果。
實驗材料和器具:
樣品:大鼠腳掌200毫克
儀器:高通量組織研磨儀(上海凈信,JXFSTPRP-24)
耗材:研磨鋼罐(上海凈信),研磨珠(上海凈信)
試劑:液氮若干
實驗步驟:
1.準備好研磨管,將研磨管和研磨球清洗干凈,并滅菌滅酶;
2.將需要研磨的組織樣品放入準備好的研磨管中,加入研磨珠。蓋好蓋子;
3.將裝有組織樣品的研磨管放入液氮中,冷凍至不沸騰為止;
4.取出已經(jīng)冷凍的適配器,把冷凍好的不銹鋼研磨管取出插入適配器中;
5.將適配器裝入凈信研磨儀中;
6.設(shè)定好參數(shù),啟動儀器至研磨程序結(jié)束,可得到粉末狀樣品。
實驗結(jié)果
Previous:全自動組織研磨機破碎酵母細胞實驗-上海凈信
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