動(dòng)物組織RNA的提取,采用傳統(tǒng)研磨方法效果不佳,難以達(dá)到后續(xù)實(shí)驗(yàn)要求,如:加入液氮后于研磨缽中手工研磨、快速勻漿器等。只有對(duì)組織進(jìn)行充分研磨才能保證RNA及蛋白的有效提取,進(jìn)而為熒光定量PCR、Western-blot等實(shí)驗(yàn)的進(jìn)一步開(kāi)展提供可靠的實(shí)驗(yàn)材料。證實(shí)上海凈信的組織研磨破碎儀可有效處理該類組織,能夠達(dá)到實(shí)驗(yàn)的要求。
動(dòng)物組織標(biāo)本樣
實(shí)驗(yàn)步驟
步驟1:把采集的臨床標(biāo)本剪切成小塊;
步驟2 :將樣本放入研磨管中,再轉(zhuǎn)移至凈信研磨儀適配器;
步驟3:設(shè)置參數(shù):研磨頻率,時(shí)間,打開(kāi)研磨管可見(jiàn)樣本已被充分研磨成黏稠狀;
步驟4:收集樣本凍存或進(jìn)行后續(xù)實(shí)驗(yàn)。
實(shí)驗(yàn)后續(xù)結(jié)果
提取的RNA及蛋白濃度較手動(dòng)研磨方式有極大提高,可完全滿足后續(xù)實(shí)驗(yàn)需求。
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