1)時(shí)間:2014/6/17 注:部分請與廠家工程師聯(lián)系
2)樣品:芹菜莖部組織
3)儀器:主機(jī),Tissuelyser動(dòng)物組織研磨器(上海凈信實(shí)業(yè)有限公司生產(chǎn))
4)配件:離心管和凈信研磨珠
4)步驟:
a.取芹菜莖部組織樣品,均勻地剪切成少量樣品塊,分別放入若干離心管中。
b.每個(gè)試管中放入凈信研磨珠和微量的裂解液,分別蓋緊管蓋后,再置入研磨適配器中,將適配器固定在研磨儀中。
c.設(shè)置Tissuelyser植物組織研磨儀的時(shí)間和頻率參數(shù)。啟動(dòng)研磨器。
d.研磨完全停止后,拿出研磨好的離心管,打開管蓋,取出研磨好的芹菜莖部組織樣品
5)結(jié)果:
研磨后的芹菜莖部組織成很均細(xì)的泥漿狀,說明用Tissuelyser植物組織研磨儀研磨芹菜莖部組織效果良好,可以用于接下來的實(shí)驗(yàn)。
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