客戶簡介:
中南民族大學(xué)位于湖北省武漢市,是中華人民共和國國家民族事務(wù)委員會(huì)直屬高校,為中國“少數(shù)民族骨干計(jì)劃”資格高校、全國深化創(chuàng)新創(chuàng)業(yè)教育改革示范高校、中國高校行星科學(xué)聯(lián)盟、湖北省“國內(nèi)一流大學(xué)建設(shè)高?!薄?/p>
實(shí)驗(yàn)?zāi)康模?/strong>
檢測藻細(xì)胞內(nèi)總蛋白含量和抗氧化酶活性
實(shí)驗(yàn)材料和器具:
樣品:微囊藻7806 (Microcystis aeruginosa PCC 7806)
工具:小美超微量超聲波細(xì)胞破碎儀(XM-650T)
耗材:10ml離心管
實(shí)驗(yàn)步驟:
1.準(zhǔn)備小美超聲波破碎儀,配加長變幅桿
2.將探頭放入準(zhǔn)備好的樣品中處理中
3.超聲條件設(shè)置
4.具體實(shí)驗(yàn)流程:取20ml集胞藻6803藻液(OD0.6-0.8),常溫離心,棄上清。沉淀加2ml的tris-HCl(40mM, PH8.0)及20ul的PMSF(100mM),超聲處理。超聲后,4度離心,取上清轉(zhuǎn)移至新的離心管,用于蛋白定量和抗氧化酶活性檢測。
注意:
1、每次破碎樣品時(shí)盡量避免探頭貼壁,也可根據(jù)客戶自己操作調(diào)節(jié)
2、由于連續(xù)超聲破碎,會(huì)使樣品溫度升高,需要客戶冰浴處理
實(shí)驗(yàn)過程、結(jié)果圖示:
XM-650T
圖1破碎前,圖2破碎后
此款儀器為上海凈信實(shí)業(yè)發(fā)展有限公司旗下小美超聲生產(chǎn)的全新超聲波破碎儀一體機(jī),是一種特殊的、快速的、高效率的一體機(jī)破碎系統(tǒng)??梢栽谳^短的時(shí)間內(nèi)完成對(duì)各種樣品的研磨、粉碎、混合及細(xì)胞破壁,以保留樣品活性。
產(chǎn)品簡介:
1、藥物提取,細(xì)胞,細(xì)菌,病毒組織破碎。如細(xì)胞內(nèi)含物的萃取。
2、物質(zhì)顆粒的分散、勻質(zhì),及乳化。如納米材料的分散。
3、加速溶解,加速化學(xué)反應(yīng)。如用于化學(xué)合成,此超聲設(shè)備配隔音箱支架、專用隔音箱、低溫系統(tǒng)。
4、超聲破碎是聲化學(xué)設(shè)備的一種應(yīng)用,可用于植物提取,水處理、固液系分散、液體中顆粒的解團(tuán)聚、促進(jìn)固液反應(yīng)等效果,使較大粒徑的聚集體分散為較小的顆粒。穩(wěn)定化指保證粉體顆粒在液體中保持長期的均勻分散等。
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