實驗?zāi)康?關(guān)于土壤中所含微生物細(xì)胞內(nèi)容物的提取。
實驗地點(diǎn): 浙江省農(nóng)業(yè)科學(xué)院土肥所。
實驗材料和器具:
樣品:干燥土壤粉末或顆粒
儀器:全自動樣品快速研磨儀(上海凈信)、金屬適配器
耗材:土壤中微生物細(xì)胞內(nèi)容物提取試劑盒(試劑、配好研磨珠的離心管)
實驗步驟
1、稱量干燥土壤粉末或顆粒;
2、將稱量好的樣品加入到試劑盒所配離心管中(圖1);
3.、先加入試劑,然后加入裂解液在渦旋儀上初步混勻;
4、設(shè)定好運(yùn)行參數(shù),將樣品管放入適配器中;
5、啟動儀器至研磨程序結(jié)束,可得到研磨后樣品(圖2);
6、將樣品放入離心機(jī)中幾分鐘后(圖3),吸取上清液進(jìn)儀器分析
實驗結(jié)果:
圖1:樣品加入后;圖2:凈信研磨儀研磨后;圖3:離心過后待提上清
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