一、研究原理
果實發(fā)育及表觀遺傳學(xué):番茄是世界范圍內(nèi)的重要蔬菜作物,同時也因為其較小的基因組,完整的遺傳圖譜以及較短的生命周期被作為研究果實生長成熟的模式植物。
對于番茄果實成熟的研究不僅能幫助了解果實成熟的機制,也對提高番茄果實品質(zhì),以及其他肉質(zhì)果實品質(zhì)具有重要的作用。近年來,番茄的果實成熟被越來越廣泛深入地研究,果實成熟的調(diào)控機制也越來越清晰。
最新研究顯示,DNA甲基化可能是轉(zhuǎn)錄因子和植物激素之外的第三個最重要的調(diào)節(jié)果實成熟的因子。然而,DNA甲基化參與調(diào)控果實成熟的機制則缺乏相應(yīng)的深入研究。
二、實驗地點
中國科學(xué)院上海植物逆境生物研究中心朗瞾博課題組
三、研磨方法
實驗步驟:
1,準(zhǔn)備好待研磨的番茄樣品2個,鋼罐,液氮,清洗并滅菌;
2,將樣品置于鋼罐中,同時將直徑的研磨鋼珠放入鋼罐中,將其整體侵入液氮幾分鐘之后并安裝到上海凈信研磨儀上;
3,設(shè)置頻率為,時間,開機進行研磨;
4,待機子停止運行,取出研磨鋼罐。得出的實驗結(jié)果如下:
研磨對比:
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