研磨心肌組織實驗目的:
研磨心肌組織,更有效快速的提取核酸蛋白。
實驗地點:中南大學湘雅醫(yī)學院孝騫樓護理實驗室461
實驗材料和器具:
樣品:小鼠心肌組織
儀器:全自動樣品快速研磨儀(上海凈信,JXFSTPRP-24)
耗材:專用研磨管(上海凈信),研磨珠(上海凈信)
試劑:PBS緩沖液
研磨心肌組織實驗步驟:
1.準備好研磨管,將研磨管和研磨球清洗干凈,并滅菌滅酶;
2.將需要研磨的心肌組織樣品剪一塊,放入準備好的研磨管中,加入幾顆研磨珠,蓋好蓋子;放到適配器中,適配器可以事先放在冰箱先預冷,在干磨過程中相對的控制溫度。
3.將適配器裝入凈信研磨儀中;
4.設定好參數(shù),啟動儀器至研磨程序結束。
研磨心肌組織實驗結果:
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