近年來,隨著生命科學(xué)研究的發(fā)展,尤其是在完成擬南芥 (Arabidopsis thaliana) 和水稻 (Oryza sativa) 等植物的基因組測序后,植物生物學(xué)發(fā)生了翻天覆地的變化。人們已經(jīng)把目光從基因的測序轉(zhuǎn)移到了基因的功能研究。在研究 DNA 的基因組學(xué)、mRNA 的轉(zhuǎn)錄組學(xué)及蛋白質(zhì)的蛋白組學(xué)后,接踵而來的是研究代謝物的代謝組學(xué) (Hall et al.,2002)。代謝組學(xué)的概念來源于代謝組,代謝組是指某一生物或細胞在一特定生理時期內(nèi)所有的低分子量代謝產(chǎn)物,代謝組學(xué)則是對某一生物或細胞在一特定生理時期內(nèi)所有低分子量代謝產(chǎn)物同時進行定性和定量分析的一門新學(xué)科 (Goodacre,2004)。它是以組群指標(biāo)分析為基礎(chǔ),以高通量檢測和數(shù)據(jù)處理為手段,以信息建模與系統(tǒng)整合為目標(biāo)的系統(tǒng)生物學(xué)的一個分支。
實驗步驟:
步驟1:將采集的各種植物組織(根莖葉,根瘤菌等)裝入研磨管,加入研磨珠。放入液氮中進行低溫處理。
步驟2:將低溫處理過的樣品放入凈信研磨儀,設(shè)置頻率,研磨完成后可以得到粉末狀的樣品
步驟3:從凈信研磨儀中取出樣品,將樣品溶解做后續(xù)實驗
實驗結(jié)果
研磨效果
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