(還可以提取如沉積物,排泄物,廢水,雪水等樣品的DNA)土壤微生物基因組DNA抽提試劑盒/提取試劑盒
(利用硅吸附DNA原理)
一,說明
本試劑盒可以快速有效的直接從土壤分離微生物基因組DNA。用上海凈信科技(全自動樣品快速勻漿機)FSTPRP儀器(將樣品與緩沖液加入帶有特殊小珠的樣品管/離心管)在幾十秒內(nèi)就可以裂解土壤中的植物、動物組織、細菌、藻類、真菌孢子、酵母、線蟲。經(jīng)過本試劑盒的分離純化,所得的DNA可以用于PCR、限制性酶切、電泳和其他使用。
濃縮SEWS-M使用前加入無水乙醇,并在瓶子上做好標(biāo)記,蓋好瓶蓋
WASH D使用前加入無水乙醇,并在瓶子上做好標(biāo)記,蓋好瓶蓋
用ddH20(滅菌)完全溶解RNA酶,將RNA酶放入水中煮,水沸后再煮沸幾十分種,并掉炎,緩慢冷至室溫后,將RNA酶取出,全部加入 sodium phosphate buffer中,充分混勻后,將sodium phosphate buffer buffer 放入保存。
試劑盒組成
實驗步驟
需了解具體步驟,請致電廠家
Previous:tissuelyser組織研磨機對提取小鼠尾巴RNA的實驗步驟
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