實驗目的
對大量和小量酵母菌分別進行研磨,進而提取其核酸或蛋白。
實驗原理
通過研磨珠的撞擊,充分破碎酵母菌細胞壁,使其內(nèi)容物流出,進而提取目標成分。
實驗材料和器具
樣品:酵母菌
儀器:全自動樣品冷凍研磨儀(上海凈信,Tissuelyser-24)
耗材:離心管,不銹鋼研磨珠(上海凈信,中號研磨珠)
實驗步驟
需了解具體步驟,請致電廠家
實驗注意事項
1.少量酵母菌研磨好后,實驗室需要檢驗離心管中酵母是否研磨達到要求,可以采用重疊離心管離心方法:具體步驟為,取一管研磨好裝有酵母的離心管,在底部用針或者其他尖銳東西扎一小孔,再在該離心管底部套一新離心管,一起離心。最后將新離心管中得到的酵母液進行鏡檢,若鏡檢無完整酵母細胞,則研磨效果達到要求。
2.如果上述步驟檢測效果不理想,可以在第一步裝樣本前,先把酵母樣本放入離心機,稍微離心,去除上浮液,再繼續(xù)實驗。
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