實(shí)驗(yàn)?zāi)康?/strong>
通過提取藥材的DNA,進(jìn)行DNA條形碼分子對(duì)照,進(jìn)而鑒定中藥。實(shí)驗(yàn)原理
通過對(duì)照藥材自身獨(dú)有的DNA條形碼記錄,可以準(zhǔn)確鑒定出中藥材品種。用DNA條形碼分子法鑒定中藥是藥典中一項(xiàng)重要的鑒定方法。
實(shí)驗(yàn)材料和器具
樣品:甘草,陳皮,阿膠飲片
儀器:高通量組織研磨儀(上海凈信,Tissuelyser-24)
耗材:不銹鋼研磨罐,研磨珠(上海凈信)
實(shí)驗(yàn)步驟
需了解具體步驟,請(qǐng)致電廠家
注意事項(xiàng)
1. 研磨罐和研磨珠需充分清洗干凈并烘干備用;若需提取藥材的RNA,則研磨罐和破碎珠要確保滅酶;
2. 每個(gè)研磨罐大約可研磨適量大小的藥材量(具體根據(jù)樣品本身的緊密度會(huì)有變動(dòng),一般樣品體積不超過研磨罐容積的三分之二);
3. 如果放入的樣品沒有脫水或者是水分含量較大的藥草類,需用液氮預(yù)冷,具體操作是:把裝好樣品和研磨珠的研磨罐浸入液氮中,直至液氮不沸騰,再迅速將研磨罐放入研磨儀中,設(shè)置參數(shù)單位振頻,間歇幾十秒后啟動(dòng)研磨儀,就能得到較好的研磨效果。較堅(jiān)硬的樣品無需液氮冷凍,如阿膠、鹿茸等。
研磨效果
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