條形酵母是將酵母通過(guò)小孔注入到裝有液體氮的氣罐中壓縮制成的。將有篩子的扣入到裝有球狀酵母的破碎罐的頂部,經(jīng)過(guò)離心破碎變成了條狀物。壓力是由人工用自由活塞注射器注入罐內(nèi),條狀可以在-80C°下被保存。
使用上海凈信Tissuelyser全自動(dòng)樣品快速研磨儀對(duì)酵母進(jìn)行研磨,將不銹鋼鋼珠和樣本分別裝入研磨鋼罐中,然后放入到用聚苯乙烯盒子盛放的液氮中,當(dāng)預(yù)冷凍過(guò)程結(jié)束后,時(shí)間一分鐘左右,或者直到原來(lái)沸騰的液體氮恢復(fù)平靜,將破碎罐從液氮中取出,并要將破碎罐沾有的液體全部清理干凈,以避免在破碎過(guò)程中發(fā)生爆炸。研磨鋼罐最好用坩堝鉗或大鑷子夾取出來(lái),而且通常要帶經(jīng)過(guò)冷凍的手套,凍傷以免。
研磨鋼罐中酵母量不要太多,否則可能會(huì)研磨不充分,留有殘?jiān)?,一般為研磨鋼罐?/3左右為宜,把剛從液氮中取出的鋼罐安裝在凈信Tissuelyser全自動(dòng)樣本快速研磨儀中,分幾次去進(jìn)行研磨,去設(shè)置頻率和時(shí)間參數(shù),每次間隔都把研磨罐放入液氮中冷卻。
在每次破碎后都要將破碎罐取下,放入到液氮中進(jìn)行冷凍,當(dāng)液氮不在沸騰時(shí),可以將鋼罐取出,破碎可以繼續(xù)進(jìn)行。
經(jīng)過(guò)100秒的破碎后,我們通過(guò)顯微鏡觀察可以發(fā)現(xiàn)95%的酵母已經(jīng)達(dá)到破碎效果,用于下一步的實(shí)驗(yàn)過(guò)程。剩余的酵母可以儲(chǔ)存,備用。
研磨鋼罐和鋼珠都可重復(fù)利用,破碎罐和破碎球可以用溫的乙醇清洗,放到干燥處貯存。
上海凈信實(shí)業(yè)發(fā)展有限公司,是目前國(guó)內(nèi)唯一家生產(chǎn)水平式組織研磨機(jī),垂直式組織研磨機(jī),三維一體式組織研磨機(jī)具有全系列生產(chǎn)線的生產(chǎn)廠家。同時(shí)還給多家國(guó)外品牌及國(guó)內(nèi)企業(yè)提供OEM,貼牌代加工服務(wù)。
中國(guó)多樣品/高通量研磨機(jī)廠家!
凈信,就是研磨機(jī)?。。?/strong>
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