1)時(shí)間:2014/9/11
2)樣品:枸杞
3)儀器:主機(jī),Tissuelyser動(dòng)物組織研磨器(上海凈信實(shí)業(yè)有限公司生產(chǎn))
4)配件:離心管和凈信研磨珠珠
4)步驟:
A.將提前準(zhǔn)備好的枸杞分裝在離心管中。
b.每個(gè)試管中放入研磨珠和PBS裂解液,分別蓋緊管蓋后,再置入研磨適配器中,將適配器固定在研磨儀中。
c.將Tissuelyser植物組織研磨儀的參數(shù)按要求設(shè)置好。啟動(dòng)研磨器。
d.研磨完全停止后,拿出研磨好的離心管,打開管蓋,取出研磨好的枸杞組織樣品。
5)結(jié)果:
研磨后的枸杞組織成很均細(xì)的泥漿狀,說明用Tissuelyser植物組織研磨儀研磨枸杞組織效果非常好,可以用于下一步實(shí)驗(yàn)。
上海凈信的全自動(dòng)樣品快速研磨儀處理與手工液氮研磨處理后提取 FT 的 DNA、RNA 在濃度、純度上均無顯著性差異 。但手工研磨有較大的樣本損失率,對(duì)于DNA提取濃度較低的樣品來說,自動(dòng)研磨儀可以做到樣品零損失,在DNA提取上有著明顯的優(yōu)勢(shì),且手工處理的過程復(fù)雜、易交叉污染,操作所用時(shí)間等方面均不及上海凈信的全自動(dòng)樣品快速研磨儀,因此自動(dòng)研磨儀對(duì)于DNA提取工作相對(duì)于手工研磨處理有很大優(yōu)勢(shì)。
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