1)時(shí)間:2014/03/10 注:部分請與廠家工程師聯(lián)系
2)樣品:水稻葉片
3)儀器:主機(jī),Tissuelyser植物組織研磨器(上海凈信實(shí)業(yè)有限公司生產(chǎn))
4)配件:離心管和凈信研磨珠
5)步驟:
A.取水稻葉片組織,用剪刀剪成若干段,分別放入離心管中。
b.每個離心管中放入幾顆凈信研磨珠和7微量的裂解液,分別將離心管蓋蓋好后,再置入研磨適配器中,將適配器固定在研磨儀中。
c.設(shè)置Tissuelyser植物組織研磨儀的研磨時(shí)間和頻率的參數(shù)。啟動研磨器。
d.研磨完全停止后,拿出適配器上的離心管,打開管蓋,取出研磨好的水稻葉片樣品。
6)結(jié)果:
由圖可以清晰的看出,研磨后的水稻葉片組織很均細(xì)的勻漿狀,說明用Tissuelyser植物組織研磨儀玫瑰花瓣效果非常良好,可以用于后續(xù)轉(zhuǎn)基因檢測工作。
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